Dr. Beng Guat Ooi

Professor

Dr. Beng Guat Ooi
615-898-2076
Room 3081, Science Building (SCI)
MTSU Box 68, Murfreesboro, TN 37132

Degree Information

  • PHD, Monash University (1988)
  • BS, Monash University (1983)

Areas of Expertise

Study of cigarette smoke mutagenicity via the analysis of DNA adducts resulting from tobacco toxicants

Investigation of the influence of yeast enzymes on wine composition and the transformation of anthocyanins during wine aging

Determining the relationships of wine and non-wine yeast strains by DNA sequence analysis of the rDNA genes and interspacer regions

Comparison of the fermentation by-products of wine and non-wine yeast

Study the effects of di...

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Study of cigarette smoke mutagenicity via the analysis of DNA adducts resulting from tobacco toxicants

Investigation of the influence of yeast enzymes on wine composition and the transformation of anthocyanins during wine aging

Determining the relationships of wine and non-wine yeast strains by DNA sequence analysis of the rDNA genes and interspacer regions

Comparison of the fermentation by-products of wine and non-wine yeast

Study the effects of different isomeric forms of 2,3-butanediol on the quality of wine

Study the inhibitory effects of xylitol and other polyols on the growth of cavities causing bacteria, Streptococcus mutans

Development of methods for the analysis of yeast metabolites produced by the transformation of phenanthrene

Studying the effects of glucose on the yield of xylitol produced from biomass-derived products by yeast

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Biography

Professional Organization Memberships
Tennessee Academy of Science

American Chemical Society (ACS)

American Society for Testing and Materials (ASTM)

Publications

  1. D. Dutta, B. G. Ooi, N. S. Chong. Major harmful effects of chemicals found in e-cigarette emissions; in E-Cigarettes: Perspectives, Regulation and Health Effects; Nova Science Publishers, Hauppauge, NY, USA. (Accepted on May 19th, 2020).
  2. N. S. Chong, D. Dutta, and B. G. Ooi.  Formation of Melamine-Derived Particles in Aqueous and Biological Matrices In An Introduction to Melamine. Ashley Harris Ed.; Nova Science Publishers, Inc. Hauppauge, NY 11788 USA.
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  1. D. Dutta, B. G. Ooi, N. S. Chong. Major harmful effects of chemicals found in e-cigarette emissions; in E-Cigarettes: Perspectives, Regulation and Health Effects; Nova Science Publishers, Hauppauge, NY, USA. (Accepted on May 19th, 2020).
  2. N. S. Chong, D. Dutta, and B. G. Ooi.  Formation of Melamine-Derived Particles in Aqueous and Biological Matrices In An Introduction to Melamine. Ashley Harris Ed.; Nova Science Publishers, Inc. Hauppauge, NY 11788 USA.
  3. B. G. Ooi, D. Dutta, K. Kazipeta, N. S. Chong. Influence of E-cigarette Emission Profile by the Ratio of Glycerol to Propylene Glycol in E-Liquid Composition. ACS Omega 2019, 4, 133338-133348.
  4. Y. E. Ejorh, W.H. Ilsley, B. G. Ooi. Elucidating the Chemisorption Phenomena in SERS Studies via Computational Modeling. Optics and Photonics Journal 2018, 8, 212-234.
  5. B. G. Ooi and S. A. Branning. Correlation of conformational changes and protein degradation with loss of lysozyme activity due to chlorine dioxide treatment. Applied Biochemistry and Biotechnology 2017, 182(2), 782-791.
  6. N. S. Chong, K. Donthula, R. A. Davies, W. H. Ilsley, B. G. Ooi. Significance of chemical enhancement effects in surface-enhanced Raman scattering (SERS) signals of aniline and aminobiphenyl isomers. Vibrational Spectroscopy 2015, 81, 22-31.
  7. A. A. Banach and B. G. Ooi. Enhancing the Yields of Phenolic Compounds during Fermentation Using Saccharomyces cerevisiae Strain 96581. Food and Nutrition Sciences 2014, 5, 2063-2070
  8. R. A. Davies, N. S. Chong, B. G. Ooi. Chemical enhancement of the Surface Enhanced Raman Scattering signals of anilines via their ortho-substituents. Optics and Photonics Journal 2013, 3 (5A), 13-23.
  9. N. S. Chong, K. A. Smith, S. Setti, B. G. Ooi. Application of gold and silver colloidal nanoparticles for the surface-enhanced Raman spectrometric analysis of melamine and 4-aminobiphenyl. International Journal of Environmental Technology and Management 2013, 16 (1/2), 3-20.
  10. K. A. Smith, N. S. Chong, K. Donthula, B. G. Ooi. Influence of the structural characteristics of silver and gold nanoparticles on the surface-enhancement factors of the Raman signals from aromatic amine. NanoFormulation 2011 Conference Proceedings 2012, 121-132.
  11. B. G. Ooi, A. L. Rambo, M. A. Hurtado. Overcoming the recalcitrance for the conversion of kenaf pulp to glucose via microwave-assisted pre-treatment processes. International Journal of Molecular Sciences 2011, 12, 1451-1463.
  12. J. Boachie, L. Bolin, N. S. Chong, and B.G. Ooi. 2010 Quantitative determination of oxygenate additives in gasoline and diesel fuels by infrared spectroscopy. 239th ACS National Meeting & Exposition 55 (1), 645-647.
  13. L. Bolin, F. Ahmed, P. Schine, B.G. Ooi, and N. S. Chong. 2010 Effects of oxygenate additives in reducing air toxics from the exhaust emissions of generators fueled by diesel and gasoline. 239th ACS National Meeting & Exposition 55 (1), 658-659.
  14. B. G. Ooi, L. Zhao, and N. S. Chong. 2009 Application of a versatile substrate coated with silver nanoparticles for surface-enhanced vibrational spectroscopy American Laboratory, 41 (7), 36-38.
  15. B. G. Ooi and Kevin R. Lankford. 2009 Strategy for adapting wine yeast for bioethanol production. International Journal of Molecular Sciences 10, 385-394.
  16. B. G. Ooi and M. J. Sanger. 2009 “Which pathway am I?” Using a game approach to teach students about biochemical pathways. Journal of Chemical Education 86: 454-455.
  17. B. G. Ooi, L. E. Wanamaker, B. M. Markuszewski, and N. S. Chong. 2008 Genetic and enological analysis of selected Saccharomyces cerevisiae strains for wine production. International Journal of Food Science Technology 43: 1111- 1120.
  18. B. G. Ooi. Technical challenges in the biomass pretreatment technologies for cellulosic bioethanol fuel. Wiley Encyclopedia of Energy (Accepted in December 2007)
  19. B. G. Ooi. 2005 Application of commercial and web-based software programs for DNA sequence analysis in undergraduate Biochemistry laboratory. Chemical Educator 10: 1-4.
  20. B. G. Ooi. 2004 DNA amplification by PCR and analysis by gel electrophoresis – An undergraduate Biochemistry experiment. Chemical Educator 9: 97-101.
  21. Ngee-Sing Chong, Omobola Oladipupo, Vichuda Hunter, Beng-Guat Ooi. 2004 Application of the AMDIS mass spectral deconvolution algorithm for the characterization of cigarette smoke. Proceedings of the 52nd ASMS Conference on Mass Spectrometry and Allied Topics, ThPD 059.
  22. B. G. Ooi, A. M. Mulisa, H. Y.  Kim, & N. S. Chong. 2003 Methods development for the detection of trace metabolites from biotransformation of polycyclic aromatic hydrocarbons by yeast.  Journal of the Tennessee Academy of Science 78 (3): 65-75.
  23. B. G. Ooi, T. T. B. Le, & B. M. Markuszewski. 2002 The effects of glucose on the yeast conversion of xylose into xylitol by C. guilliermondii and C. tropicalis. Electronic Journal of Environmental, Agricultural, and Food Chemistry 1(3).
  24. B. G. Ooi & L. K. Miller. 1991 The Influence of antisense RNA on transcription mapping of the 5' terminus of a baculovirus RNA. Journal of General Virology, 72, 527- 534
  25. X. Wang, B. G. Ooi & L. K. Miller. 1991 Baculovirus vectors for multiple gene expression and for occluded virus production. Gene, 100, 131-137
  26. J. R. Lupski, Y. H. Zhang, M. Rieger, M. Minter, B. Hsu, B. G. Ooi, T. Koeuth & E. R. B. McCabe.  1990 Mutational analysis of the Escherichia coli glpFK region with Tn5 mutagenesis and the polymerase chain reaction.  Journal  of Bacteriology, 172, 6129-6134
  27. B. G. Ooi & L. K. Miller. 1990 Transcription of the baculovirus polyhedrin gene reduces the levels of an antisense transcription initiated downstream. Journal of Virology, 64, 3126-3129 
  28. B. G. Ooi, C. Rankin & L. K. Miller. 1989 Downstream sequence augment transcription from the essential initiation site of a baculovirus polyhedrin gene. Journal of Molecular Biology, 210, 721-736
  29. C. Rankin, B. G. Ooi & L. K. Miller. 1988 Eight base pairs encompassing the transcription start point are the major determinant for baculovirus polyhedrin gene expression. Gene, 70, 39-49
  30. B. G. Ooi & L. K. Miller. 1988 Regulation of host RNA levels during baculovirus infection. Virology, 166, 515-523
  31. B. G. Ooi, H. B. Lukins, A. W. Linnane &  P. Nagley. 1987    Biogenesis of mitochondrial 66. A mutation in the 5'-untranslated region of yeast mitochondrial oli1 mRNA leading to impairment in translation of subunit 9 of the mitochondrial ATPase complex. Nucleic Acids Research, 15, 1965-1977
  32. B. G. Ooi & P. Nagley. 1986 The oli gene and flanking sequences in mitochondrial DNA of Saccharomyces cerevisiae: The complete nucleotide sequence of a 1.35 kilobase petite mitochondrial DNA genome covering the oli1 gene. Current Genetics, 10, 713-723
  33. P. Nagley, R. M. Hall & B. G. Ooi. 1986 Amino acid substitutions in mitochondrial ATPase subunit 9 of S.  cerevisiae leading to oligomycin or venturicidin resistance. FEBS Letters, 195, 159-163
  34. A. W. Linnane, H. B. Lukins, P. Nagley, S. Marzuki, R. G. Hadikusumo, M. J. B. Jean-Francois, U. P. John, B. G. Ooi, L. C. Watkins, T. A. Willson, J. Wright & S. Meltzer. 1985 Assembly of yeast mitochondrial H+-ATPase: Correlative studies involving gene sequencing and immunochemical probes of assembly. In Achievements and Perspectives in Mitochondrial Research, Volume I: Bioenergetics, (F. Palmieri, ed.), Elsevier/ North Holland Biomedical Press, Amsterdam, 211-222
  35. B. G. Ooi, C. E. Novitski & P. Nagley. 1985 DNA sequence analysis of the oli1 gene reveals amino acid changes in mitochondrial ATPase subunit 9 from oligomycin resistant mutants of Saccharomyces cerevisiae. European Journal of Biochemistry, 152,709-714
  36. B. G. Ooi, G. L. McMullen, A. W. Linnane, P. Nagley & C. E. Novitski.  1985  Biogenesis of mitochondrial 62. DNA sequence analysis of mit- mutations in the mitochondrial oli1 gene coding for mitochondrial ATPase subunit 9 in Saccharomyces cerevisiae. Nucleic Acids Research, 13, 1327-1339
  37. I. G. Macreadie, C. E. Novitski, R. J. Maxwell, U. John, B. G. Ooi, G. L. McMullen, H.B. Lukins, A. W. Linnane & P. Nagley. 1983   Biogenesis of mitochondrial 59. The mitochondrial ATPase subunit 8 in Saccharomyces cerevisiae. Nucleic Acids Research, 11, 4435-4451 

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Presentations

Rachel E. Thornell and Beng Guat Ooi. "The Sugar that Exhibits Dental Health Benefits"; The 11 th Annual Undergraduate Research Symposium of the College of Basic And Applied Sciences. (April 15, 2005)

S. B. Naquin, Z. T. Frensley, N. S. Chong & B. G. Ooi. "A Study of Bidi Cigarette Smoke on Salmonella typhimurium strains TA97a and TA98"; SCHOLAR'S DAY 2004: A University-Wide Showcase of Research, Scholarship, Creativity, and Public Service. (October 22, 2004)

Ngee-Si...

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Rachel E. Thornell and Beng Guat Ooi. "The Sugar that Exhibits Dental Health Benefits"; The 11 th Annual Undergraduate Research Symposium of the College of Basic And Applied Sciences. (April 15, 2005)

S. B. Naquin, Z. T. Frensley, N. S. Chong & B. G. Ooi. "A Study of Bidi Cigarette Smoke on Salmonella typhimurium strains TA97a and TA98"; SCHOLAR'S DAY 2004: A University-Wide Showcase of Research, Scholarship, Creativity, and Public Service. (October 22, 2004)

Ngee-Sing Chong, Omobola Oladipupo, Vichuda Hunter, Beng-Guat Ooi. "Application of the AMDIS Mass Spectral Deconvolution Algorithm for the Characterization of Cigarette Smoke"; 52nd American Society of Mass Spectrometry Conference in Nashville, TN (May 27, 2004)

Rachel E. Thornell and Beng Guat Ooi. "The Sugar that Exhibits Dental Health Benefits"; The Golden Goggle Lecture & Research Open House (April 27, 2004)

S. B. Naquin, Z. T. Frensley, N. S. Chong & B. G. Ooi. "A Study of Bidi Cigarette Smoke on Salmonella typhimurium strains TA97a and TA98";, 2003 Southeast Regional ACS meeting in Atlanta, GA (November 16-19, 2003)

S. B. Naquin, Z. T. Frensley, N. S. Chong & B. G. Ooi. "A Study of Bidi Cigarette Smoke on Salmonella typhimurium strains TA97a and TA98";, Tennessee Academy of Science 113th meeting in conjuction with the Tennessee Science Teachers Association in Flanklin, TN (November 13-15, 2003)

B. G. Ooi, T. T. B Le., & B. M. Markuszewski. "The effects of glucose on the yeast conversion of xylose into xylitol by C. guilliermondii and C. tropicalis";, The University-wide showcase for Faculty Research and Creativity (May 2, 2003)

B. G. Ooi, T. T. B Le., & B. M. Markuszewski. "The effects of glucose on the yeast conversion of xylose into xylitol by C. guilliermondii and C. tropicalis";, The Golden Goggle Lecture & Research Open House (April 24, 2003)

B. G. Ooi, T. T. B Le., & B. M. Markuszewski. "The effects of glucose on the yeast conversion of xylose into xylitol by C. guilliermondii and C. tropicalis";, Pittsburgh Conference (March, 2003)

N. S. Chong, H. Y. Kim, A. M. Mulisa, and B. G. Ooi, "Methods Development for the Detection of Trace Metabolites from the Biodegradation of Polycyclic Aromatic Hydrocarbons by Yeasts";, Pittsburgh Conference (March, 2003)

H. Y. Kim, N.S. Chong, and B.G. Ooi. "Methods Development for the Detection of Trace Metabolites from the Biodegradation of Polycyclic Aromatic Hydrocarbons by Yeasts";. Third MTSU University-wide Faculty Research Symposium, (April 25, 2002)

B. M. Bednarz and B.G. Ooi. " Relationships between Wine and Non-Wine Yeast Strains Based on Restriction Enzyme Analyses of Genomic and mtDNA";. The Golden Goggle Lecture & Research Open House (April 16, 2002)

H. Y. Kim, N.S. Chong, and B.G. Ooi. "Methods Development for the Detection of Trace Metabolites from the Biodegradation of Polycyclic Aromatic Hydrocarbons by Yeasts";. Second Joint Meeting of Tennessee Academy of Science and the Kentucky Academy of Science, (November 8-10, 2001).

A. M. Mulisa, B.G. Ooi & N. S. Chong. "Chromatographic Characterization of Metabolites from the Biotransformation of Polycyclic Aromatic Hydrocarbons by C. tropicalis CP1-1";. The Second MTSU University-wide Faculty Research Symposium, (April 25, 2001).

B. M. Bednarz & B.G. Ooi. "Comparative Studies of Wine Composition Produced by Different Yeast Strains";. The Golden Goggles Lecture/ Research Open House, (April 23, 2001). Poster was selected for first prize.

B. G. Ooi, N.S. Chong, and A.M. Mulisa. "Biodegradation of Polycyclic Aromatic Hydrocarbons by Yeasts";. The Golden Goggles Lecture/Research Open House, (April 23, 2001).

B. M. Bednarz & B.G. Ooi. "Comparative Studies of Wine Composition Produced by Different Yeast Strains";. The Sixth Annual Undergraduate Research Symposium, (April 12, 2001).

A. M. Mulisa, B.G. Ooi & N. S. Chong. "Chromatographic Characterization of Metabolites from the Biotransformation of Polycyclic Aromatic Hydrocarbons by C. tropicalis";. Pittsburgh Conference, (March, 2001).

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Awards

  1. Excellence in Publications; 2008-2009; College of Basic & Applied Sciences
  2. Ciba Award for biofuel poster presentation in nationwide competition; June 2011; American Chemical Society Green Chemistry and Engineering Conference
  3. Two graduate poster awards –Second and Third Place; 2012; Scholars’ week
  4. One Second Place undergraduate poster award; 2012; Scholars’ week
  5. One Third Place undergraduate oral presentation; 2015;12th Annual TLSAMP  Conference
  6. One Second Place undergraduate poster award; 2016; Scholar’s week

Research / Scholarly Activity

Dissertation/Thesis
Master of Science Thesis entitled "Methods Development for Dectection of Trace Metabolites from the Biotransformation of Phenanthrene by Candida tropicalis 96745 CP1-1". Alex M. Mulisa